LOCUS       40924_26486        3793 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pK19mob DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Okamoto S, Niki H.
  TITLE     NBRP cloning vector collection sequence project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima, 
            Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 3793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics, 
            Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka 
            411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             313..1104
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     oriT            complement(1445..1554)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        complement(1789..1879)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(2073..2163)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2523..3111
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3399..3420
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3435..3465
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3473..3489
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3497..3513
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(3526..3582)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3583..3599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
       61 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
      121 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
      181 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
      241 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga
      301 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
      361 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
      421 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
      481 ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
      541 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
      601 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
      661 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
      721 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
      781 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
      841 cggatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
      901 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
      961 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     1021 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     1081 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     1141 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     1201 gcttcggaat cgttttccgg gacgccctcg cggacgtgct catagtccac gacgcccgtg
     1261 attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc
     1321 gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg
     1381 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     1441 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     1501 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gccccgctga
     1561 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     1621 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     1681 gcggaaaagc gctgcttccc tgctgttttg tggaatatct accgactgga aacaggcaaa
     1741 tgcaggaaat tactgaactg aggggacagg cgagagacga tgccaaagag ctcctgaaaa
     1801 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1861 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     1921 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     1981 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
     2041 tttgaggtgc tccagtggct tctgtttcta tcagctcctg aaaatctcga taactcaaaa
     2101 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     2161 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     2221 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     2281 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     2341 ggcttctgtt tctatcaggg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     2401 ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2461 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2641 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2881 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     2941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3361 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3421 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3481 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg
     3541 caggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca
     3601 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3661 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3721 cagcctgaat ggcgaatggc gcgataagct agcttcacgc tgccgcaagc actcagggcg
     3781 caagggctgc taa
//
